Endothelial lesions within the transplanted kidney are a major determinant of chronic allograft nephropathy. Epoxyeicosatrienoic acids (EETs) are endothelium-derived hyperpolarizing factors with anti-inflammatory, antiproliferative and vasodilator properties. The main goal of the investigators' study is to evaluate whether genetic polymorphisms of specific enzymes responsible for the bioavailability of EETs are associated with post-transplant kidney function. To this end, 80 kidney transplant recipients will be included. Prespecified genetic polymorphisms of CYP 2J2, CYP 2C8, CYP 2C9, CYP 2C9, CYP 2C19 and EPHX2 will be determined. Kidney function will be recorded 3, 6, 12 and 36 months after transplantation. Flow-mediated dilatation, EETs and circulating biomarkers of endothelial function will be measured in the radial artery. The expected results of this study to provide preliminary evidence supporting a beneficial role of an increase in the bioavailability of EETs in kidney transplant recipients.
Name: Blood sampling
Description: Blood sampling is done for Kidney transplant recipients for evaluation of the polymorphisms and EETs dosageType: ProcedureKidney transplant recipients
Name: flow-mediated distal stimulation of the forearm radial artery by cutaneous heating
Description: Flow-mediated distal stimulation of the forearm radial artery by cutaneous heating for Kidney transplant recipientsType: ProcedureKidney transplant recipients
Description: eGFR Scores range from 0 [kidney failure] to >90 [normal function]
Measure: Difference between patients with the EPHX2 Lys55Arg polymorphism and patients without in estimated Glomerular Filtration Rate (eGFR) Time: 12 Months after transplantationDescription: eGFR Scores range from 0 [kidney failure] to >90 [normal function]
Measure: Difference between patients with the EPHX2 Lys55Arg polymorphism and patients without in estimated Glomerular Filtration Rate (eGFR) Time: 3 Months after transplantationDescription: eGFR Scores range from 0 [kidney failure] to >90 [normal function]
Measure: Difference between patients with the EPHX2 Lys55Arg polymorphism and patients without in estimated Glomerular Filtration Rate (eGFR) Time: 6 Months after transplantationDescription: eGFR Scores range from 0 [kidney failure] to >90 [normal function]
Measure: Difference between patients with the EPHX2 Lys55Arg polymorphism and patients without in estimated Glomerular Filtration Rate (eGFR) Time: 36 Months after transplantationDescription: eGFR Scores range from 0 [kidney failure] to >90 [normal function]
Measure: Difference between patients with specified polymorphisms and patients without in estimated Glomerular Filtration Rate (eGFR) Time: 3 Months after transplantationDescription: eGFR Scores range from 0 [kidney failure] to >90 [normal function]
Measure: Difference between patients with specified polymorphisms and patients without in estimated Glomerular Filtration Rate (eGFR) Time: 6 Months after transplantationDescription: eGFR Scores range from 0 [kidney failure] to >90 [normal function]
Measure: Difference between patients with specified polymorphisms and patients without in estimated Glomerular Filtration Rate (eGFR) Time: 12 Months after transplantationDescription: eGFR Scores range from 0 [kidney failure] to >90 [normal function]
Measure: Difference between patients with specified polymorphisms and patients without in estimated Glomerular Filtration Rate (eGFR) Time: 36 Months after transplantationDescription: EEts plasma concentration is measured using liquid chromatography coupled to tandem mass spectrometry before and after distal cutaneous heating. Unit is ng per mL
Measure: Difference between blood EETs concentration at baseline and after distal cutaneous heating for patients with the specified polymorphisms. Time: Day 1Description: EEts plasma concentration is measured using liquid chromatography coupled to tandem mass spectrometry before and after distal cutaneous heating. Unit is ng per mL
Measure: Difference between blood EETs concentration at baseline and after distal cutaneous heating for patients without the specified polymorphisms. Time: Day 1Description: Variation of diameter of the radial artery is measured in millimeter.
Measure: Difference between variation of diameter of the forearm radial artery after distal cutaneous heating for patients with the specified polymorphisms. Time: Day 1Description: Variation of diameter of the radial artery is measured in millimeter.
Measure: Difference between variation of diameter of the forearm radial artery after distal cutaneous heating for patients without the specified polymorphisms. Time: Day 1Single Group Assignment
There is one SNP
Difference between patients with the EPHX2 Lys55Arg polymorphism and patients without in estimated Glomerular Filtration Rate (eGFR). --- Lys55Arg ---