SNPMiner Trials by Shray Alag


SNPMiner Trials: Clinical Trial Report


Report for Clinical Trial NCT01351324

Developed by Shray Alag, 2019.
SNP Clinical Trial Gene

Acute Elevation of Non-esterified Fatty Acids on Endothelial Function and Insulin Sensitivity: A Comparison of Saturated and Long Chain n-3 Polyunsaturated Fatty Acids During the Postprandial Phase

Experimental elevation of non-esterified fatty acids (NEFA) impairs endothelial function and insulin sensitivity but the impact of NEFA composition is unknown. The objective was to test the effect of acute elevation of NEFA enriched with either saturated fatty acids (SFA) or SFA with long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) on postprandial vascular function measured via flow-mediated dilatation (FMD), laser Doppler iontophoresis (LDI) and digital volume pulse (DVP), followed by a hyperinsulinaemic-euglycaemic clamp as a measure of whole body insulin sensitivity.

NCT01351324 Cardiovascular Risk Factors
MeSH: Insulin Resistance
HPO: Insulin resistance

1 Interventions

Name: Absence or presence of fish oil

Description: Oral dose of saturated fat with or without fish oil and a heparin infusion

Type: Dietary Supplement

SFA SFA + LC n-3 PUFA


Primary Outcomes

Measure: Flow-mediated dilatation

Time: Change from baseline to 240 min

Measure: Flow-mediated dilatation

Time: Change from 240 min to 390 min

Secondary Outcomes

Measure: Digital volume pulse

Time: Change from baseline to 240 min

Measure: Laser Doppler iontophoresis

Time: Change from baseline to 240 min

Measure: Insulin sensitivity

Time: 390 min

Measure: NEFA composition

Time: Change from baseline to 240 min

Measure: Circulating endothelial function markers

Time: Change from baseline to 240 min

Measure: Digital volume pulse

Time: Change from 240 min to 390 min

Measure: Laser Doppler iontophoresis

Time: Change from 240 min to 390 min

Measure: Circulating endothelial markers

Time: Change from 240 min to 390 min

Purpose: Basic Science

Allocation: Randomized

Crossover Assignment


There is one SNP

SNPs


1 E298D

Inclusion Criteria: - Healthy - Either homozygous for eNOS Glu298 (wildtype)or eNOS Asp298 (variant) Exclusion Criteria: - Smokers - Raised fasting blood lipids - Taking excessive fish oil supplements (>1g EPA/DHA per day) - Taking medication known to influence blood lipids, blood pressure or blood clotting Inclusion Criteria: - Healthy - Either homozygous for eNOS Glu298 (wildtype)or eNOS Asp298 (variant) Exclusion Criteria: - Smokers - Raised fasting blood lipids - Taking excessive fish oil supplements (>1g EPA/DHA per day) - Taking medication known to influence blood lipids, blood pressure or blood clotting Cardiovascular Risk Factors Insulin Resistance To investigate potential diet-gene interactions, potential subjects (n=370) were prospectively genotyped for the eNOS Glu298Asp polymorphism, of which 35 were Asp298 and 150 were Glu298 homozygotes. --- Glu298Asp ---



HPO Nodes


HPO:
Insulin resistance
Genes 56
BLK PPP1R3A KLF11 HNF1A HNF1B IGF1 LIPE NEUROD1 IGFALS ZMPSTE24 SLC12A3 CEL LMNA ALMS1 CLCNKB PTF1A INS PIK3R1 POLD1 FOS ABCC8 CYP19A1 CIDEC KCNJ11 AGPAT2 INSR ZFP57 WRN DCAF17 GATA6 PDX1 MFN2 APPL1 PLAGL1 AKT2 CAVIN1 BSCL2 HSD3B2 DBH GCK LMNB2 PAX4 CAV1 HSD11B1 PPARG XRCC4 NSMCE2 PLIN1 HNF4A HYMAI EIF2AK3 LEP LEPR STAT3 CEP19 PMM2