SNPMiner Trials by Shray Alag


SNPMiner Trials: Clinical Trial Report


Report for Clinical Trial NCT01713023

Developed by Shray Alag, 2019.
SNP Clinical Trial Gene

Effect of Fructose and Glucose and TAS1R2 in Glucose, Triglycerides, Uremia, Oxidative Stress, Feelings Related to Food Intake of Individuals With Type 1 Diabetes

- BACKGROUND: Individuals with diabetes report innate preference for sweet foods, possibly due to genetic variants. In addition, studies have shown that ingestion of fructose promotes lower postprandial blood glucose, compared to glucose. However, excessive intake may increase triglycerides, uric acid and oxidative stress, due to oxidative priority. - AIMS: To investigate the influence of fructose and glucose and taste receptor, type 1, member 2 (TAS1R2) in glucose, triglycerides, uremia, oxidative stress, feelings related to food intake and palatability of individuals with type 1 diabetes. - METHODS: The trial is a single-blind, two-way crossover (1-week washout) study in 30 subjects with type 1 diabetes. Blood samples were collected before and 2-hours after the participants receive 75g of fructose or 75g of glucose dissolved in 200 ml water. Capillary blood glucose were assessed at 30, 90, 120, and 180 minutes to determine glucose, and visual analogue scales for measurement of appetite sensation were assessed at 70, 120, and 190 minutes. - PURPOSE: The research proposal adds knowledge about the TAS1R2 (Ile191Val) polymorphism and around the most suitable monosaccharide for individuals with diabetes type 1.

NCT01713023 Type 1 Diabetes
MeSH: Diabetes Mellitus Diabetes Mellitus, Type 1
HPO: Diabetes mellitus Type I diabetes mellitus

2 Interventions

Name: Glucose

Description: The same described in Arm description

Type: Dietary Supplement

Glucose solution Fructose solution

Name: Fructose

Description: The same described in Arm description

Type: Dietary Supplement

Glucose solution Fructose solution


Primary Outcomes

Description: Determine the blood glucose, triglycerides, oxidation of substrates, uremia, oxidative stress, feelings related to food intake of individuals with type 1 diabetes, before and 3-hours after ingestion of 75g of fructose or 75g of glucose.

Measure: Compare the effects of fructose and glucose and TAS1R2 in postprandial metabolism of individuals with type 1 diabetes

Time: baseline up to 3 hours each day study. Total study: 2 years

Secondary Outcomes

Description: Determine the peak plasma blood glucose before and 3-hours after ingestion of 75g of fructose or 75g of glucose. We also test capillary blood glucose before and 30, 90, 120, and 180 minutes after solutions.

Measure: Effect of fructose or glucose on blood glucose

Time: Baseline, and up to 3-hours after solutions

Description: Determine the triglycerides levels before and 3-hours after ingestion of 75g of fructose or 75g of glucose.

Measure: Effect of fructose or glucose on triglycerides levels

Time: Baseline, and up to 3-hours after solutions

Description: Determine the uric acid, pyruvic acid, and lactate levels before and 3-hours after ingestion of 75g of fructose or 75g of glucose.

Measure: Effect of fructose or glucose on uric acid, pyruvic acid, and lactate

Time: Baseline, and up to 3-hours after solutions

Description: Determine the malondialdehyde level before and 3-hours after ingestion of 75g of fructose or 75g of glucose.

Measure: Effect of fructose or glucose on oxidative stress

Time: Baseline, and up to 3-hours after solutions

Description: TAS1R2 (taste receptor, type 1, member 2 T1R2) is the component specific to sweet taste perception, and does not change during the life. We will determine whether Ser9Cys (dbSNP: rs9701796) and Ile191Val (dbSNP: rs35874116)variations in TAS1R2 were associated with differences the sweet taste perceptions.

Measure: Effect of two polymorphisms in the gene TAS1R2 in the sweet taste perception

Time: only at baseline

Description: Determine the peak plasma blood glucose before and 3-hours after ingestion of 75g of fructose or 75g of glucose. We also test capillary blood glucose before and 30, 90, 120 and 180 minutes after solutions.

Measure: Effect of fructose or glucose on blood glucose

Time: Baseline, and up to 3-hours after solutions

Description: Two different visual analogue scales will be used to assess hunger, satiety, fullness, prospective food consumption, palatability, taste, aftertaste, smell, and visual appeal of the solutions. The scales will be performed at 7, 35, 95 and 120 minutes after solutions.

Measure: Effect of fructose or glucose on appetite and palatability

Time: Baseline, and up 120 minutes

Description: Determine the glucagon levels before and 3-hours after ingestion of 75g of fructose or 75g of glucose

Measure: Effect of fructose or glucose on glucagon levels

Time: Baseline, and up to 3-hours after solutions

Description: Determine the leptin levels before and 3-hours after ingestion of 75g of fructose or 75g of glucose

Measure: Effect of fructose or glucose on leptin levels

Time: Baseline, and up to 3-hours after solutions

Purpose: Health Services Research

Allocation: Randomized

Crossover Assignment


There are 2 SNPs

SNPs


1 I191V

- PURPOSE: The research proposal adds knowledge about the TAS1R2 (Ile191Val) polymorphism and around the most suitable monosaccharide for individuals with diabetes type 1. Compare the effects of fructose and glucose and TAS1R2 in postprandial metabolism of individuals with type 1 diabetes. --- Ile191Val ---

We will determine whether Ser9Cys (dbSNP: rs9701796) and Ile191Val (dbSNP: rs35874116)variations in TAS1R2 were associated with differences the sweet taste perceptions.. Effect of fructose or glucose on blood glucose. --- Ser9Cys --- --- Ile191Val ---

The Ile191Val (rs35874116) polymorphism in TAS1R2 gene will be detected by using a TaqMan allelic discrimination assay (ABI number C_55646_20; Applied Biosystems, Foster City, CA) with real-time polymerase chain reaction on an ABI 7000 Sequence Detection System (Applied Biosystems). --- Ile191Val ---


2 S9C

We will determine whether Ser9Cys (dbSNP: rs9701796) and Ile191Val (dbSNP: rs35874116)variations in TAS1R2 were associated with differences the sweet taste perceptions.. Effect of fructose or glucose on blood glucose. --- Ser9Cys ---



HPO Nodes


HPO:
Diabetes mellitus
Genes 326
SOX2 MKRN3 SOX3 MKKS SNORD115-1 SPATA7 CDKN2A HFE CERKL UBR1 PRPF31 LRAT PRSS1 TREX1 PRSS2 IFT140 ARL2BP GLRX5 SLC29A3 MAK AHI1 ABCA4 PDE4D ZBTB20 PDE6A PDE6G ZMPSTE24 IMPDH1 CEL STUB1 SPINK1 RTEL1 PDE6B LEMD3 INS HLA-DQB1 GJB4 IFT172 AMACR KIZ CDHR1 HLA-DRB1 CYP19A1 CFTR DHX38 MAFA GAS1 INSR ZFP57 MC4R GATA3 CTRC GATA6 PDX1 IPW PEX1 BBS1 PWRN1 PEX6 BBS2 DNAJC21 PEX10 CDH23 SRP54 DHDDS NDN PRPF6 GCK NDP KCTD1 AGBL5 GPR101 PTCH1 HNF4A ADAR TTPA TUB SNORD116-1 HESX1 STAT1 STAT3 TULP1 BLK BLM ARL6 IFIH1 TTC8 GJA1 PROM1 NEK2 GJB3 SAG FBN1 NEUROD1 SARS2 PNPLA6 BRCA1 ALMS1 BRAF BRCA2 PROKR2 CLCNKB TINF2 KLHL7 REEP6 FAM161A PIK3R1 GLI2 ABCC8 KCNJ11 RNASEH2C ARNT2 NPAP1 SCAPER FSCN2 DCAF17 AHR PRCD DKC1 FGF8 PLAGL1 AKT2 NOP10 FGFR1 PLCD1 GNAS PWAR1 SEMA4A HERC2 MERTK FOXH1 PLIN1 HYMAI NODAL USH2A CLRN1 CNGB1 CNGA1 EIF2AK3 MOG CDON CA4 IMPG2 PDE11A FOXP3 FOXC2 HJV PROK2 RNASEH2B KRAS TCF4 KLF11 EFL1 HNF1A BEST1 HNF1B PNPLA2 NR2E3 MAGEL2 POC1A OFD1 SLC25A4 USB1 NRL CLIP2 APOA5 CISD2 RNASEH2A CTC1 PTF1A WFS1 GPR35 POLD1 FOS RRM2B POLG AGPAT2 RBP3 WRN SLC19A2 HGSNAT GLIS3 SHH AIRE KIAA1549 FUZ BAZ1B RDH12 POMGNT1 PRPH2 ARHGEF18 DNM1L CP TWNK CPA1 CASR PCARE SAMHD1 TDGF1 AIP CAV1 PSTPIP1 FXN APOE IDH3B PPARG RFC2 XRCC4 WRAP53 SIX3 GTF2IRD1 NSMCE2 PRPF8 DLL1 NHP2 TERC TERT EDA RGR SLC7A14 AR LEP OPA1 LEPR PALB2 POLR3A RHO CRB1 ZIC2 MKRN3-AS1 CRX RLBP1 TTC7A PPP1R3A CNBP MST1 ERGIC1 LHX1 TGIF1 USP8 MLXIPL LIG4 LIMK1 EYS ARL3 LIPE OTX2 IGF1R GTF2I HMGA2 ATP6 TRNC SLC12A3 LMNA COX1 COX2 COX3 GUCA1B IFT88 CYTB SLC16A2 PRPF4 PRPF3 ELMO2 PDE8B VANGL1 SBDS EIF2S3 IER3IP1 ZNF408 CIDEC ND1 ARMC5 ND4 ND5 ND6 PRKACA PRKAR1A NKX2-5 APPL1 TRNE TRNF ROM1 ZFYVE26 PARN CAVIN1 BSCL2 TRNH RP9 RP1 ELN TRNK PAX4 RP2 TRNL1 RPGR ATM HAMP CTNS CTNNB1 C8ORF37 TRNQ TRNS1 TRNS2 POLG2 TRNV TOPORS TRNW KDSR HBB SUFU IL2RA RPE65 DNAJC3 SNRNP200 SNRPN PALLD TBL2 DISP1 EDA2R NEUROG3 TP53 CEP19 SMAD4 PCNT ZNF513
Type I diabetes mellitus
Genes 39
TTC7A MST1 AIRE DNAJC21 SRP54 TRNE TCF4 TRNF EFL1 HNF1A TRNH TRNL1 KCTD1 PSTPIP1 TRNQ TRNS1 TRNS2 SLC12A3 COX1 STUB1 COX2 COX3 TRNW CLCNKB SLC16A2 EDA EIF2AK3 GPR35 DNAJC3 INS SBDS EDA2R NEUROG3 STAT1 ND1 ND4 ND5 ND6 FOXP3