SNPMiner Trials by Shray Alag


SNPMiner Trials: Clinical Trial Report


Report for Clinical Trial NCT00187655

Developed by Shray Alag, 2019.
SNP Clinical Trial Gene

Effect of Genetic Variation in the Transporter, OAT3, on the Renal Secretion of Cefotaxime

In the proposed study, we plan to use a genotype to phenotype strategy to study the role of the organic anion transporter, OAT3, in drug response. More specifically we will examine the contribution of OAT3 to the renal clearance of anionic drugs such as cefotaxime by studying individuals with a non-functional (or poorly-functional) variant of OAT3.

NCT00187655 Focus Groups

1 Interventions

Name: Cefotaxime

Description: Cefotaxime will be administered as a single IV push of 2 grams over 5 minutes.

Type: Drug

Cefotaxime


Primary Outcomes

Description: Participants were stratified by their OCT3 genotype, heterozygous vs homozygous. The renal clearance of cefotaxime was measured by urine content of cefotaxime metabolites in participants after the single IV push administration of 2 grams of cefotaxime.

Measure: Effect of OAT3 on Renal Secretion of Cefotaxime IV Based on Genotype

Time: post dose up to 24 hours

Purpose: Health Services Research

Single Group Assignment


There are 5 SNPs

SNPs


1 A310V

One variant (A310V) which showed increased transport of estrone sulfate compared to the common allele was identified in a single Caucasian subject. --- A310V ---


2 F129L

Four variants were identified that resulted in a complete loss of function: F129L (in one Hispanic subject), R149S (in an Caucasian and an African American subject), Q239stop (in the same African American subject), and R277W (in an Asian subject). --- F129L ---


3 I305F

An additional variant (I305F) which showed reduced transport ability for some, but not all, OAT3 substrates was identified in three Asian and one Hispanic subject. --- I305F ---


4 R149S

Four variants were identified that resulted in a complete loss of function: F129L (in one Hispanic subject), R149S (in an Caucasian and an African American subject), Q239stop (in the same African American subject), and R277W (in an Asian subject). --- F129L --- --- R149S ---


5 R277W

Four variants were identified that resulted in a complete loss of function: F129L (in one Hispanic subject), R149S (in an Caucasian and an African American subject), Q239stop (in the same African American subject), and R277W (in an Asian subject). --- F129L --- --- R149S --- --- R277W ---



HPO Nodes