SNPMiner Trials by Shray Alag


SNPMiner Trials: Clinical Trial Report


Report for Clinical Trial NCT02487615

Developed by Shray Alag, 2019.
SNP Clinical Trial Gene

Subclinical Atherosclerosis: Biomarkers of Early Disease

Subclinical atherosclerosis is the atherosclerotic process identified before clinical symptoms and thus it can be a useful marker of future cardiovascular events. It can be evaluated by many methods. This study included the diagnosis of subclincal atherosclerosis by four different methods: coronary calcium score, carotid doppler ultrasound to quantify intima media thickness and carotid plaques, exercise stress test and ankle brachial index. Clinical data, anthropometric measures (body mass index, abdominal circumference), markers of inflammation (high sensitive - C reactive protein, TNF alfa and Lipoprotein Associated Phospholipase A2), fat tissue function (leptin, resistin and adiponectin), glucose metabolism (fasting plasma glucose, glycated hemoglobin and insulin) and genetics markers of atherosclerotic process were evaluated as biomarkers of subclinical atherosclerosis in a uneventful population.

NCT02487615 Primary Prevention
MeSH: Atherosclerosis
HPO: Atherosclerosis Type IV atherosclerotic lesion


Primary Outcomes

Description: Subclinical atherosclerosis evaluation by quantifying intima media thickness by Doppler

Measure: Carotid intima-media thickness (cIMT)

Time: Four Weeks

Secondary Outcomes

Description: Subclinical atherosclerosis evaluation by Coronary Artery Calcium by CT

Measure: Coronary artery calcium score

Time: Four weeks

Description: Subclinical atherosclerosis evaluation by brachial ankle index (BAI) by blood pressure measurement

Measure: Brachial Ankle Index

Time: Four weeks

Description: Myocardial ischemia investigation by treadmill exercise test

Measure: Ischemia induced by exercise stress test

Time: Four weeks

Description: Blood samples for Adiponectin, resistin, leptin, tumor necrosis factor alpha (TNF-α), lipoprotein associated phospholipase A2 (Lp-PLA2) dosing

Measure: Adiponectin, resistin, leptin, tumor necrosis factor alpha (TNF-α), lipoprotein associated phospholipase A2 (Lp-PLA2)

Time: Four weeks

Description: DNA and extraction from leukocytes

Measure: Genotyping of DNA at the following rs2383206, rs10757274, rs10757278, rs1051931, rs16874954 and rs1799724 and RNA of PLA2G7 (2-ΔCT), TNF-α (2-ΔCT), LEP (2-ΔCT), LEPR (2-ΔCT)

Time: Four weeks

Time Perspective: Cross-Sectional

Cohort


There are 6 SNPs

SNPs


1 rs1051931

Genotyping of DNA at the following rs2383206, rs10757274, rs10757278, rs1051931, rs16874954 and rs1799724 and RNA of PLA2G7 (2-ΔCT), TNF-α (2-ΔCT), LEP (2-ΔCT), LEPR (2-ΔCT).

Blood sample was also stored for DNA (rs2383206, rs10757274, rs10757278, rs1051931, rs16874954 and rs1799724) and RNA PLA2G7 (2-ΔCT), TNF-α (2-ΔCT), LEP (2-ΔCT), LEPR (2-ΔCT) extraction and analyses.


2 rs10757274

Genotyping of DNA at the following rs2383206, rs10757274, rs10757278, rs1051931, rs16874954 and rs1799724 and RNA of PLA2G7 (2-ΔCT), TNF-α (2-ΔCT), LEP (2-ΔCT), LEPR (2-ΔCT).

Blood sample was also stored for DNA (rs2383206, rs10757274, rs10757278, rs1051931, rs16874954 and rs1799724) and RNA PLA2G7 (2-ΔCT), TNF-α (2-ΔCT), LEP (2-ΔCT), LEPR (2-ΔCT) extraction and analyses.


3 rs10757278

Genotyping of DNA at the following rs2383206, rs10757274, rs10757278, rs1051931, rs16874954 and rs1799724 and RNA of PLA2G7 (2-ΔCT), TNF-α (2-ΔCT), LEP (2-ΔCT), LEPR (2-ΔCT).

Blood sample was also stored for DNA (rs2383206, rs10757274, rs10757278, rs1051931, rs16874954 and rs1799724) and RNA PLA2G7 (2-ΔCT), TNF-α (2-ΔCT), LEP (2-ΔCT), LEPR (2-ΔCT) extraction and analyses.


4 rs16874954

Genotyping of DNA at the following rs2383206, rs10757274, rs10757278, rs1051931, rs16874954 and rs1799724 and RNA of PLA2G7 (2-ΔCT), TNF-α (2-ΔCT), LEP (2-ΔCT), LEPR (2-ΔCT).

Blood sample was also stored for DNA (rs2383206, rs10757274, rs10757278, rs1051931, rs16874954 and rs1799724) and RNA PLA2G7 (2-ΔCT), TNF-α (2-ΔCT), LEP (2-ΔCT), LEPR (2-ΔCT) extraction and analyses.


5 rs1799724

Genotyping of DNA at the following rs2383206, rs10757274, rs10757278, rs1051931, rs16874954 and rs1799724 and RNA of PLA2G7 (2-ΔCT), TNF-α (2-ΔCT), LEP (2-ΔCT), LEPR (2-ΔCT).

Blood sample was also stored for DNA (rs2383206, rs10757274, rs10757278, rs1051931, rs16874954 and rs1799724) and RNA PLA2G7 (2-ΔCT), TNF-α (2-ΔCT), LEP (2-ΔCT), LEPR (2-ΔCT) extraction and analyses.


6 rs2383206

Genotyping of DNA at the following rs2383206, rs10757274, rs10757278, rs1051931, rs16874954 and rs1799724 and RNA of PLA2G7 (2-ΔCT), TNF-α (2-ΔCT), LEP (2-ΔCT), LEPR (2-ΔCT).

Blood sample was also stored for DNA (rs2383206, rs10757274, rs10757278, rs1051931, rs16874954 and rs1799724) and RNA PLA2G7 (2-ΔCT), TNF-α (2-ΔCT), LEP (2-ΔCT), LEPR (2-ΔCT) extraction and analyses.



HPO Nodes


HPO:
Atherosclerosis
Genes 64
NPC1 COL3A1 TGFB2 TGFB3 TGFBR1 LDLRAP1 TGFBR2 HGD TGFBR3 MFAP5 ANGPTL6 ABCA1 MYH11 LIPC FBN1 ZMPSTE24 CSF2RA MYLK CSF2RB GLB1 LMNA ALMS1 SC5D CYP7A1 LOX ENPP1 MAT2A FOS KCNJ5 ESR1 CYP27A1 ACTA2 AGPAT2 AGXT WRN ANTXR1 OAS1 APOA1 NPC2 SMPD1 APOB CAVIN1 BSCL2 ELN PRKG1 CAV1 LCAT APOE PPARG ENG TNXB SERPIND1 LDLR BANF1 ABCC6 ABCG5 ABCG8 GGCX NOTCH3 ZNF687 SMAD3 PCSK9 CEP19 FOXE3
Type IV atherosclerotic lesion
Genes 1
APOE