The purpose of this study is to determine whether the outcome of interferon therapy on HCV infected patients can be early precisely predicted with a novel mathematic method with Chinese population.
Name: Interferon Alfa-2b, Ribavirin
Description: Interferon:dosage,5 million units/person;frequency,every other day (qod);duration,48 weeks;Subcutaneous injection. Ribavirin: dosage,15mg/kg/day;frequency,three times a day (t.i.d);duration,48 weeks;take orally.Type: DrugInterferon and ribavirin
Description: Blood HCV RNA copies were assayed with Roche - COBAS® AmpliPrep/COBAS® TaqMan® HCV Test.
Measure: Blood HCV RNA Copies Time: 0h,8h,10h,12h,18h,24h,37h,43h,3d,7d,2w,4w,6w,12w,24w,48wDescription: IL28 gene polymorphism,rs8099917,rs12979860,etc
Measure: IL-28B polymorphism Time: BaselineDescription: During the first 3 days, blood samples are collected for PBMC separation and microarray analysis.
Measure: Microarray Analysis of PBMC Gene Expression Time: Baseline,8h,18h,3dDescription: HCV NS5A is cloned into T vector and sequenced for evolutionary analysis.
Measure: HCV genotype Time: BaselineDescription: Co-infection status are analyzed.
Measure: Blood Anti-HCV,HBV Antibody Time: BaselineDescription: Deep sequencing is used for blood serum HCV genome analysis.
Measure: HCV genome sequencing Time: 0h,8h,10h,12h,18h,24h,37h,43h,3dDescription: ALT AST are assayed to detect the hepatic function.
Measure: Alanine Aminotransferase (ALT) and Aspartate transaminase (AST) Time: Baseline,4w,6w,12w,24w,48wDescription: Fibrosis is analyzed with Fibroscan.
Measure: Fibrosis stage Time: Baseline,4w,12w,24w,48wDescription: The distribution and absolute count of the different types of blood cells are assayed.
Measure: Regular blood test Time: Baseline,4w,12w,24w,48wDescription: Electrocardiography is taken to avoid severe side effects.
Measure: Electrocardiography Time: Baseline,4w,12w,24w,48wDescription: Patients are asked whether they take alcohol or smoke cigarettes during the therapy period.
Measure: Alcohol ,smoking condition Time: Baseline,4w,12w,24w,48wDescription: Patients will be asked about their drug usage history.
Measure: Drug abuse history Time: BaselineCohort
There are 2 SNPs
IL28 gene polymorphism,rs8099917,rs12979860,etc.
IL28 gene polymorphism,rs8099917,rs12979860,etc.